Flow cytometry is an essential tool in modern diagnostic and research laboratories, widely used for immunophenotyping, cell sorting, and analyzing the physical and chemical characteristics of cells. This course offers a thorough, structured journey into the principles and practices of flow cytometry, combining theory with real-world clinical applications.

Designed by expert faculty and enriched with rare and complex case studies, this course empowers participants to build a solid foundation in flow cytometry and develop the skills needed to interpret simple and complex cases confidently. Emphasis is placed on clinical relevance, ensuring that learners understand both the technical execution and the diagnostic implications of flow cytometry findings.

Participants will learn to design panels, analyze multi-parameter data, and apply this knowledge to the diagnosis and monitoring of hematologic malignancies, immunodeficiencies, and other disease states.

Key Learning Outcomes:

By the end of the course, participants will be able to:

• Understand the basic principles and instrumentation of flow cytometry

• Master sample preparation techniques and staining protocols

• Develop and optimize antibody panels for immunophenotyping

• Interpret flow cytometry data with confidence using gating strategies and software

• Recognize flow patterns in hematologic malignancies and immunologic disorders

• Understand the role of flow cytometry in minimal residual disease (MRD) detection

• Apply quality control and assurance practices to ensure accurate, reproducible results

• Correlate flow cytometry findings with clinical and morphological data

Course Highlights:

• Foundational Modules: Basics of flow cytometry, fluorochromes, laser principles, and cell markers

• Clinical Focus: Applications in hematologic malignancies, PNH, immunodeficiencies, plasma cell dyscrasias, and more

• Expert-Led Case Discussions: Rare and illustrative real-world cases presented by practicing hematopathologists

• Troubleshooting Sessions: Common pitfalls and how to avoid them in daily lab practice

• Assessment & Certification: Quizzes, interactive case reviews, and a certificate upon successful completion